DNase I, RNase-free

• Catalog Number: EN401-02
• Category: Tools
• Description: eoxyribonuclease I (DNase I) is an endonuclease that digests single- or double-stranded DNA. It recognizes and cleaves phosphodiester bonds to produce a single deoxynucleotide or single- or double-stranded oligodeoxynucleotide with 5'-phosphorylated and 3'-hydroxylated ends. The activity of DNase I depends on Ca2+, and DNase I can also be activated by divalent metal ions such as Mg2+ and Mn2+. In the presence of Mg2+, DNase I nonspecifically recognizes and cleaves a double-stranded DNA at any site on either strand, and in the presence of Mn2+, it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with one or two nucleotide overhangs.
• Storage: Store at -30 ~ -15°C and transport at ≤0°C
• Note: For research use only
• Application notes: High activity Very low RNase residue Good stability
• Expiration Date: 12 months from date of receipt.