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Item 1 of 4
DNAJC6 Antibody (Center)
Catalog Number: orb1927602
| Catalog Number | orb1927602 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB24673 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 99997 Da |
| Target | This DNAJC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-281 amino acids from the Central region of human DNAJC6. |
| UniProt ID | O75061 |
| NCBI | NP_001243793.1, NP_055602.1, NP_001243794.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Putative tyrosine-protein phosphatase auxilin, Dna Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
DNAJC6 Antibody (Center) western blot analysis in K562 (lane 1), HL-60 cell line (lane 2) and mouse brain tissue (lane 3) lysates (35 ug/lane).This demonstrates the DNAJC6 antibody detected the DNAJC6 protein (arrow).
DNAJC6 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
DNAJC6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJC6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-DNAJC6 Antibody (Center) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lane 3: U-87 MG whole cell lysate. Lane 4: HL-60 whole cell lysate. Lane 5: mouse cerebellum lysate. Lane 6: mouse brain lysate. Lane 7: rat cerebellum lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 100 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
