DNA Ligase 1 antibody

• Catalog Number: orb339024
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LIG1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Ligase 1. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: LIG1
• Entrez: 3978
• UniProt ID: P18858
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti DNA ligase 1 antibody, anti DNA ligase I anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DNA Ligase 1 expression in 22RV1 (A), MCF7 (B) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 133 kD)

Immunohistochemical analysis of DNA Ligase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.