DNA Damage ELISA Kit

SKU: orb511110

Description

Assess DNA damage by measuring 8-hydroxy-2-deoxyguanosine in urine, cell culture, plasma and other sample matrices. This kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze using an absorbance plate reader.

Images & Validation

• Application Notes:
  1. Prepare samples and controls in 1X Assay Buffer or buffer of choice (0.5 mL at a cell density of 5 x 105 – 1 x 106 cells/mL)., 2. Reconstitute Total ROS Green with 100 µL DMSO to produce a 500X stock concentrate., 3. Dilute 1:10 by adding 900 µL 1X Assay Buffer to the 500X stock Total ROS Green to produce a 50X working solution., 4. Add Total ROS Green to each sample at 1:50. For example, add 10 µL into 490 µL cultured cells., 5. Incubate 1 hour at 37°C., 6. Treat cells with test compounds for desired period of time. Keep cells protected from light., 7. Analyze with a flow cytometer. Total ROS Green excites at 490 nm and emits at 520 nm.

Key Properties

• Sample Types: Culture medium, urine, plasma/serum, cell lysates, tissue samples, saliva

Storage & Handling

• Storage: 8-OHdG at -20°C, other components at 2-8°C
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

8-hydroxy-2-deoxyguanosine (8-OHdG)