DNA/ BstEII Digest

• Catalog Number: orb65515
• Category: Tools
• Description: The BstEII digest of λ DNA yields the following discrete fragments (in base pairs): 8453*, 7242, 6369, 5687*, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117. The cohesive ends (12b cos site of bacteriophage) of fragments 8453 bp and 5687 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.
• Concentration: 200 ng/µl
• Form/Appearance: liquid (supplied in Jena Bioscience 6x Green Gel Loading Buffer (#PCR-254-gr))
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Preparation: Lambda DNA (48.502 bp) was completely digested by BstEII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. ; Recommended Load: 5 µl per lane.
• Expiration Date: 12 months from date of receipt.