DLL3 antibody

• Catalog Number: orb527267
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DLL3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23871
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IF: 1:25, IHC-P: 1:50-100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 64618
• Target: DLL3
• UniProt ID: Q9NYJ7
• NCBI: NP_058637.1, NP_982353.1
• Alternative names: Delta-like protein 3, Drosophila Delta homolog 3,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with orb527267 at 1:25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG(green).The nuclear counter stain is DAPI (blue).

Immunofluorescent analysis of U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with orb527267 at 1:25 dilution.Secondary antibody was Dylight® 488-conjugated goat anti-rabbit IgG(green).

Western blot:All lanes:Anti-DLL3 Antibody (C-term) at 1:1000 dilution.Lane 1:A375;Lane 2:U-251 MG;Lane 3:NCI-H1299;Lane 4:Human brain lysate;Lane 5:Mouse brain lysate;Lane 6:Rat brain lysate.

Western blot:All lanes:Anti-DLL3 Antibody (C-term) at 1:1000 dilution.Lane 1:NCI-H1299;Lane 2:Human brain tissue lysate;Lane 3:Mouse brain tissue lysate;Lane 4:Rat brain.

Western blot:All lanes:Anti-DLL3 Antibody (C-term) at 1:2000 dilution.Lane 1:A375;Lane 2:human brain lysate;Lane 3:human liver lysate.

Immunohistochemical analysis of human brain tissue using orb527267 performed on the Leica® BOND RXm at 1:500 dilution.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of human testis tissue using orb527267 performed on the Leica® BOND RXm at 1:500 dilution.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.