DLin-KC2-DMA

SKU: orb1308377

Description

DLin-KC2-DMA is an ionizable cationic lipid utilized for siRNA delivery in lipid nanoparticles (LNPs). It enables effective gene silencing, such as targeting GAPDH, in both in vitro and in vivo research applications for therapeutic development.

Research Area

Metabolism Research

Key Properties

• CAS Number: 1190197-97-7
• MW: 642.09
• Purity: 98.60% (May vary between batches)
• Formula: C₄₃H₇₉NO₂
• SMILES: CCCCC\C=C/C\C=C/CCCCCCCCC1(CCCCCCCC\C=C/C\C=C/CCCCC)OCC(CCN(C)C)O1
• Target: Liposome,Others
• Solubility: Ethanol:50 mg/mL (77.87 mM);DMSO:100 mg/mL (155.74 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (6.23 mM)

Bioactivity

• In Vivo:
  METHODS: C57Bl6 mice were injected with DLinKC2-DMA-formulated LNP siRNA (5 mg/kg, tail vein injection) via the tail vein to study the in vivo gene silencing properties of DLinKC2-DMA-formulated LNP siRNA. RESULTS DLinKC2-DMA effectively silenced target genes in APcs in vivo.
• In Vitro:
  METHODS: Macrophages (MΦ) and dendritic cells (DC) were treated with DLinKC2-DMA (1,5μg/mL) to observe the effect of DLinKC2-DMA on siRNA-mediated gene silencing. GAPDH and control α-tubulin expression were evaluated using Western blot analysis and flow cytometry. RESULTS At a dose level of 5μg/ml, LNPs containing DLinKC2-DMA were again the most effective gene silencing agents.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DLin KC2 DMA, DLinKC2DMA, DLin-KC-2-DMA, DLin-KC2-DMA, inhibit, Inhibitor

Chemical structure of orb1308377

Compound Identifiers

PubChem CID

44464860