DKK4 antibody

• Catalog Number: orb18362
• Category: Antibodies
• Description: Goat polyclonal antibody to DKK4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 24.9
• Target: DKK4
• Entrez: 27121
• Protein Sequence: RQHARLRVCQKIEKL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DKK4 antibody, anti dickkopf homolog 4 (Xenop
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30-35kDa band seen in Jurkat lysate. Recommended for use at 1-3 μg/ml. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32kDa species of DKK4.
• Expiration Date: 12 months from date of receipt.

orb18362 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.