DKK1 antibody

• Catalog Number: orb18888
• Category: Antibodies
• Description: Goat polyclonal antibody to DKK1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse
• Concentration: 10 μg/ml
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 28.7
• Target: DKK1
• Entrez: 22943
• Protein Sequence: CDHHQASNSSRLHT
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DKK1 antibody, anti SK antibody, anti DKK-1 a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 38kDa band observed in human spleen and bone marrow lysates after 1 μg/ml antibody staining. Please note that a band of similar size is reported in the literature (PMID 10383463). However, the calculated size is 28.7kDa according to NP_36374. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18888 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb18888 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb18888 (10µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb18888 (1µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of Human Bone Marrow lysate using DKK1 antibody

Immunohistochemical staining of Human Placenta using DKK1 antibody