DIG-11-ddUTP

SKU: orb65286

Description

Digoxigenin-11-ddUTP is enzymatically incorporated into the 3'-End of oligonucleotides. It acts as a chain terminator leading to the addition of one Digoxigenin label per molecule only. The resulting Digoxigenin-labeled oligonucleotide probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups: 10:1.

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λmax 290 nm, ε 13.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 1048.91 g/mol (free acid); Detected MW: 1048.32 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₃H₆₃N₄O₂₀P₃
• SMILES: O=C(NCCCCCC(=O)NCC#Cc1c(=O)[nH]c(=O)n([C@@H]2O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)CC2)c1)CO[C@H]1CC[C@@]2([C@H]3C[C@@H](O)[C@]4(C)[C@@H](C5=CC(=O)OC5)CC[C@]4(O)[C@@H]3CC[C@@H]2C1)C

Storage & Handling

• Storage: store at -20 °C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproyl-[5-(3-aminopropargyl) -2',3'-dideoxyuridine-5'-triphosphate], Triethylammoniumsalt

Compound Identifiers

PubChem CID

171337514