Dicer/DICER1 Antibody

• Catalog Number: orb234271
• Category: Antibodies
• Description: Dicer/DICER1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 218682 MW
• UniProt ID: Q9UPY3
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Endoribonuclease Dicer;3.1.26.3;Helicase with RNas
• Note: For research use only
• Application notes: WB: The detection limit for Dicer is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of MCF-7 cells using anti-Dicer/DICER1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody.Lane 1:human HeLa cell;2:human K562 cell;3:human HepG2 cell;4:human 293T cell;5:rat C6 cell;6:mouse brain tissue;7:mouse NIH/3T3 cell.

IHC analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody. Dicer/DICER1 was detected in a paraffin-embedded section of human breast cancer tissue.

IHC analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody. Dicer/DICER1 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue.

IHC analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody. Dicer/DICER1 was detected in a paraffin-embedded section of human cervical cancer tissue.