DHX37 Rabbit Polyclonal Antibody

SKU: orb183102

Description

DHX37 Rabbit Polyclonal Antibody

Research Area

DNA / RNA Binding, DNA and RNA Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DHX37 (51-150/1157aa)
• Target: DHX37
• Molecular Weight: 129 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DDX37; DEAH(Asp-Glu-Ala-His) box polypeptide 37; DEAH box protein 37; FLJ41974; Gm1050; DHX37_HUMAN; Gm451; KIAA1517; MGC2695; MGC4322; MGC46245; MGC90028; mKIAA1517; Probable ATP dependent RNA helicase DHX37.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DHX37) Polyclonal Antibody, Unconjugated (orb183102) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T Cell (Human) Lysate at 40 ug, Primary: Anti-DHX37 (orb183102) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 129kD, Observed band size: 135kD.

Quick Database Links

Gene Symbol

DHX37

UniProt

Q8IY37