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Catalog Number | orb1788357 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This DFFB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from the Central region of human DFFB. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Mouse, Rat |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Immunogen | 203-238 aa |
UniProt ID | O76075 |
MW | 39110 Da |
Tested applications | FC, IHC-P, WB |
Dilution range | WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | DNA fragmentation factor subunit beta, 3---, Caspa Read more... |
Note | For research use only |
Western blot analysis of lysates from A431, Hela cell line (from left to right), using DFFB Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded M.kidney section using DFFB Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H.kidney section using DFFB Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using DFFB Antibody (Center) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.