DFFA Antibody

• Catalog Number: orb1239883
• Category: Antibodies
• Description: DFFA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45.The immunogen is located within the last 50 amino acids of DFF45.
• Concentration: 1 mg/ml
• Dilution range: DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: DFFA
• UniProt ID: O00273
• NCBI: NP_004392
• Storage: DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H
• Note: For research use only
• Application notes: DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.

Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 µg/mL.

Immunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 µg/mL. Green: DFF45 Antibody (orb1239883) Blue: DAPI staining.