Dffa Antibody

• Catalog Number: orb1239701
• Category: Antibodies
• Description: Dffa Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: ICAD antibody was raised against a 20 amino acid peptide near the carboxy terminus of mouse ICAD.The immunogen is located within the last 50 amino acids of ICAD.
• Concentration: 1 mg/ml
• Dilution range: ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: Dffa
• UniProt ID: O54786
• NCBI: O54786
• Storage: ICAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ICAD Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ICAD Antibody: ICAD, DFF35, Dff45, ICAD-L, ICAD-S,
• Note: For research use only
• Application notes: ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry of ICAD in mouse kidney tissue with ICAD antibody at 5 µg/ml.

Immunofluorescence of ICAD in mouse kidney tissue with ICAD antibody at 5 µg/ml. Green: ICAD antibody (orb1239701) Red: Phylloidin staining Blue: DAPI staining.