desmosome/hemidesmosome Antibody [F12], Rabbit IgG

• Catalog Number: orb1671860
• Category: Antibodies
• Description: desmosome/hemidesmosome Antibody [F12], Rabbit IgG
• Clonality: Recombinant
• Clone Number: F12
• Tested applications: IEM, IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12. A431 cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r-chimeric version of F12 (orb1671860 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.