Desmoglein 2/DSG2 Antibody

• Catalog Number: orb614112
• Category: Antibodies
• Description: Desmoglein 2/DSG2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Desmoglein 2/DSG2 recombinant protein (Position: L24-E1020).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 160 kDa
• UniProt ID: Q14126
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Desmoglein-2; Cadherin family member 5; HDGC; DSG2
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IF analysis of DSG2 using anti-DSG2 antibody.

Flow Cytometry analysis of HEPA 1-6 cells using anti-DSG2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U87 cells using anti-DSG2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of RH35 cells using anti-DSG2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IHC analysis of DSG2 using anti-DSG2 antibody.

IHC analysis of DSG2 using anti-DSG2 antibody.

IHC analysis of DSG2 using anti-DSG2 antibody.

WB analysis using anti-DSG2 antibody.Lane 1:placenta tissue, Lane 2:K562 cell, Lane 3:HeLa cell, Lane 4:U2OS cell, Lane 5:HepG2 cell, Lane 6:PC-3 cell.