Desmoglein 1 Antibody / DSG1

• Catalog Number: orb1824875
• Category: Antibodies
• Description: Recognizes a protein of ~150kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: DSG1/8576R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant human DSG1 protein was used as the immunogen for the Desmoglein 1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This Desmoglein 1 antibody is available for research use only.
• UniProt ID: Q02413
• Storage: Aliquot the Desmoglein 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human skin tissue with Desmoglein 1 antibody (clone DSG1/8576R). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.