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Catalog Number | orb420121 |
---|---|
Category | Proteins |
Description | Deoxyribonuclease I |
Form/Appearance | Liquid (sterile filtered) |
Purity | Deoxyribonuclease I is Lyophilized in vials. Each 10,000 unit vial contains 2 mg glycine, 2 μmoles calcium, and ≥10,000 units of DNase I. Each 2,500 unit vial contains 0.5 mg glycine, 0.5 μmoles calcium, and ≥2,500 units of DNase I. Dissolving the entire 10,000 unit vial in 5 ml, or the entire 2,500 unit vial in 1.25 ml, provides the equivalent of a 1 mg/ml solution. (ku = 1000un). Store at 2 - 8°C. |
Conjugation | Unconjugated |
Target | DNASE1 |
Hazard Information | Non-Toxic |
UniProt ID | P00639 |
Biological Activity | 2,290 Kunitz/mL |
NCBI | 9913 |
Storage | Store vial at -20° C or colder prior to opening product. |
Buffer/Preservatives | See application note. |
Alternative names | Deoxyribonuclease I reagent, DNL1 Read more... |
Note | For research use only |
Application notes | Deoxyribonuclease I application is one unit will cause an increase in the absorbance at 260 nm of 0.001 per minute per ml at 25° C, pH 5.0 when acting on highly polymerized DNA according to the assay method of Kunitz (J.Gen.Physiol. 33, 349 and 363 (1950)). A solution in 1 mM Calcium Chloride and 50% (v/v) glycerol. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-Fr, IHC-P, WB | |
Equine, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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