Deoxyribonuclease I

SKU: orb420121

Description

Deoxyribonuclease I

Images & Validation

• Application Notes:
  Deoxyribonuclease I application is one unit will cause an increase in the absorbance at 260 nm of 0.001 per minute per ml at 25° C, pH 5.0 when acting on highly polymerized DNA according to the assay method of Kunitz (J.Gen.Physiol. 33, 349 and 363 (1950)). A solution in 1 mM Calcium Chloride and 50% (v/v) glycerol. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Biological Activity: 2,290 Kunitz/mL
• Target: DNASE1
• Purity: Deoxyribonuclease I is Lyophilized in vials. Each 10,000 unit vial contains 2 mg glycine, 2 μmoles calcium, and ≥10,000 units of DNase I. Each 2,500 unit vial contains 0.5 mg glycine, 0.5 μmoles calcium, and ≥2,500 units of DNase I. Dissolving the entire 10,000 unit vial in 5 ml, or the entire 2,500 unit vial in 1.25 ml, provides the equivalent of a 1 mg/ml solution. (ku = 1000un). Store at 2 - 8°C.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or colder prior to opening product.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: See application note.
• Concentration: 630 unit
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Deoxyribonuclease I reagent, DNL1

Quick Database Links

Gene Symbol

DNASE1

UniProt

P00639

RefSeq:9913