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Deoxyribonuclease I

Deoxyribonuclease I

Catalog Number: orb420121

DispatchUsually dispatched within 5-10 working days
$ 413.00
Catalog Numberorb420121
CategoryProteins
DescriptionDeoxyribonuclease I
Form/AppearanceLiquid (sterile filtered)
PurityDeoxyribonuclease I is Lyophilized in vials. Each 10,000 unit vial contains 2 mg glycine, 2 μmoles calcium, and ≥10,000 units of DNase I. Each 2,500 unit vial contains 0.5 mg glycine, 0.5 μmoles calcium, and ≥2,500 units of DNase I. Dissolving the entire 10,000 unit vial in 5 ml, or the entire 2,500 unit vial in 1.25 ml, provides the equivalent of a 1 mg/ml solution. (ku = 1000un). Store at 2 - 8°C.
ConjugationUnconjugated
TargetDNASE1
Hazard InformationNon-Toxic
UniProt IDP00639
Biological Activity2,290 Kunitz/mL
NCBI9913
StorageStore vial at -20° C or colder prior to opening product.
Buffer/PreservativesSee application note.
Alternative namesDeoxyribonuclease I reagent, DNL1
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NoteFor research use only
Application notesDeoxyribonuclease I application is one unit will cause an increase in the absorbance at 260 nm of 0.001 per minute per ml at 25° C, pH 5.0 when acting on highly polymerized DNA according to the assay method of Kunitz (J.Gen.Physiol. 33, 349 and 363 (1950)). A solution in 1 mM Calcium Chloride and 50% (v/v) glycerol. Specific conditions for reactivity should be optimized by the end user.
Expiration Date12 months from date of receipt.
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