Deoxycholic acid sodium salt

SKU: orb1302113

Description

Deoxycholic acid sodium salt

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 302-95-4
• MW: 414.56
• Purity: 99.75%
• Formula: C₂₄H₃₉NaO₄
• SMILES: [Na+].C[C@H](CCC([O-])=O)[C@H]1CCC2C3CC[C@@H]4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• Target: Endogenous Metabolite,GPCR19
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:0.5 mg/mL (1.21 mM);H2O:330 mg/mL (796.02 mM);DMSO:20 mg/mL (48.24 mM)

Bioactivity

• In Vitro:
  Deoxycholic acid (DCA) and chenoDeoxycholic acid (CDCA) ar the common ingredients of duodenal reflux that act synergistically in many physiological and pathological processes the cells are repeatedly exposed to 100 μM CDCA and Deoxycholic acid at pH 5.5 for up to 120 min. To simulate chronic local recurrent disease in vitro the gastric cancer cell line MGC803 is exposed to acidified medium (pH 5.5) containing 100 μM Deoxycholic acid and CDCA. An untreated log-growth MGC803 cell line is generated to be used as a control in normal pH media. After daily 10 min exposure to the acidified bile acids for 60 weeks, MGC803-resistant cells are able to survive and proliferate after 120 min exposure.
• Cell Research:
  MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serum and 100 U mL Penicillin and 100 mg mL Streptomycin. To generate MGC803-resistant cells the pH value o the MGC803 culture medium is adjusted to the Experimental conditions usin the hydrochloric acid (A) the bile acids GCDA and Deoxycholic acid are diluted to optimal working concentration of 100 μM (B) with culture medium, an the ove All pH (A+B) is adjusted to pH 5.5, simulatin the gastric envir nMent. Initially, MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h the Experimental time and conditions are optimized in our preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure is repeated and it takes 60 weeks fo the MGC803 cells to survive and proliferate unde the exposure of A+B for 120 min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells for 60 weeks the morphological Changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 week.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Deoxycholic acid, Deoxycholic acid sodium, Deoxycholic acid sodium salt, Endogenous Metabolite, bile acid, G protein-coupled bile acid receptor, G protein-coupled Bile Acid Receptor 1, EndogenousMetabolite, GPBAR1, GPCR19, G-protein coupled receptor 19, inhibit, Inhibitor, Sodium deoxycholate, Sodium Desoxycholate, TGR5

Compound Identifiers

PubChem CID

23693948