DENTT Antibody

• Catalog Number: orb1523753
• Category: Antibodies
• Description: DENTT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: Between 643 and 693
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: DENTT
• UniProt ID: Q9H2G4
• NCBI: NP_071400.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: differentially-expressed nucleolar TGF-beta1 targe
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human DENTT by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat cells. Antibodies: Affinity purified rabbit anti-DENTT antibody orb1523753 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DENTT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DENTT antibody orb1523753 used for IP at 6 µg/mg lysate.

Detection of human DENTT by immunohistochemistry. Samples: FFPE serial sections of human testis. Antibody: Affinity purified rabbit anti-DENTT used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human DENTT by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-DENTT used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB