Dengue NS1 Antibody

SKU: orb260202

Description

Mouse monoclonal antibody to Dengue NS1

Images & Validation

• Reactivity: Virus
• Application Notes:
  Applications: Lateral flow immunoassay. Assay Conditions: 1. 1-2 days fresh prepared gold particle with maximal homogeneity in gold particle size, λ 525-529, OD 1.2-1.4. 2. Conjugation at pH 8.5, conjugation time should be 6-8 hours. 3. Blocking condition: remove supernatant and re-suspend gold pellet bound with anti-dengue NS1 to OD 8.0- 8.2, add casein to the final 0.3-0.4%. 4. Treat sample pad with 50mM sodium borate, 0.5% mouse serum and 0.5% Triton X-100, pH8.0

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Purity: 95% pure (12% SDS-PAGE and Coomassie staining).
• Purification: Purified monoclonal IgG by protein A chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 2 vials of sterile Filtered clear colorless solution.
• Buffer/Preservatives: *Dengue NS1 Antibody solution for gold conjugation (3.6mg/ml) in PBS and Sodium nitrate. *Dengue NS1 Antibody solution for membrane coating (2.2mg/ml) in PBS and Sodium nitrate.
• Disclaimer: For research use only