Dendra2 antibody

• Catalog Number: orb699857
• Category: Antibodies
• Description: Rabbit polyclonalantibody to Dendra2
• Clonality: Monoclonal
• Clone Number: PAb (836)
• Tested applications: FC, ICC, WB
• Reactivity: All
• Isotype: Rabbit polyclonal
• Immunogen: Dendra2 tag
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Dendra2
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Dendra2 tag antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).

Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct; reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of anti-Dendra2 rabbit polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for Dendra2/GPI fusion protein at approximately 35 kDa.