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Item 1 of 3
Denatured ATG1 Antibody
Catalog Number: orb1928103
| Catalog Number | orb1928103 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB17499 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 112631 Da |
| Target | This denatured ATG1 antibody is generated from rabbits immunized with a denatured human ATG1 recombinant protein. |
| UniProt ID | O75385 |
| NCBI | NP_003556.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Serine/threonine-protein kinase ULK1, Autophagy-re Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Denatured ATG1 in mouse cerebellum tissue lysates (35 ug/lane). ATG1 (arrow) was detected using the purified Pab.
Denatured ATG1 Antibody flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Denatured ATG1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
