DEFB103B Antibody

• Catalog Number: orb1272731
• Category: Antibodies
• Description: DEFB103B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-3. Human BD-3 specific antibody was purified by affinity chromatography employing immobilized hBD-3 matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect hBD-3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.SandwichTo detect hBD-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human BD-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3. Western Blot:To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: DEFB103B
• UniProt ID: P81534
• NCBI: P81534
• Storage: BD-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: BD-3, HBD3, HBP3, DEFB3, DEFB103, BD3, DEFB103BBet
• Note: For research use only
• Application notes: ELISA:Indirect:To detect hBD-3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.SandwichTo detect hBD-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human BD-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3. Western Blot:To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hBD-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BD-3 (orb1272730) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-3.