Decorin/DCN Antibody

• Catalog Number: orb221233
• Category: Antibodies
• Description: Decorin/DCN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Decorin recombinant protein (Position: D31-K359). Human Decorin shares 80% and 77% amino acid (aa) sequences identity with mouse and rat Decorin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 39747 MW
• UniProt ID: P07585
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Decorin;Bone proteoglycan II;PG-S2;PG40;DCN;SLRR1B
• Note: For research use only
• Application notes: WB: The detection limit for Decorin is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Decorin using anti-Decorin antibody.Lane 1:human MCF-7 cell; 2:human HeLa cell; 3:human HepG2 cell; 4:human SW620 cell; 5:rat liver tissue; 6:rat RH35 cell; 7:rat PC-12 cell; 8:rat C6 cell; 9:mouse thymus tissue.

IHC analysis of Decorin using anti-Decorin antibody. Decorin was detected in a paraffin-embedded section of human mammary cancer tissue.