DEAC-dUTP

• Catalog Number: orb178360
• Category: Tools
• Description: DEAC-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substi-tute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended DEAC-dUTP/dTTP ratio for Nick Translation: 30-50% DEAC-dUTP/70-50% dTTP.
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; Color: yellow; pH: 7.5 ± 0.5
• Concentration: 1.0 mM-1.1 mM
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 766.48 g/mol (free acid); Detected MW: 766.11 g/mol (free acid)
• Application notes: Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 426 nm, λem 480 nm, ε 57.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Formula: C₂₆H₃₃N₄O₁₇P₃
• SMILES: N(C/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)C(=O)c1cc2c(oc1=O)cc(cc2)N(CC)CC
• Storage: store at -20 °C
• Alternative names: Aminoallyl-dUTP-DEAC, Diethylaminocoumarin-5-dUTP,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.