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Catalog Number | orb178360 |
---|---|
Category | Tools |
Description | DEAC-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substi-tute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended DEAC-dUTP/dTTP ratio for Nick Translation: 30-50% DEAC-dUTP/70-50% dTTP. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | filtered solution (30 kDa) in 10 mM Tris-HCl; Color: yellow; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 766.48 g/mol (free acid); Detected MW: 766.11 g/mol (free acid) |
SMILES | N(C/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)C(=O)c1cc2c(oc1=O)cc(cc2)N(CC)CC |
Formula | C26H33N4O17P3 |
Storage | store at -20 °C |
Alternative names | Aminoallyl-dUTP-DEAC, Diethylaminocoumarin-5-dUTP, Read more... |
Note | For research use only |
Application notes | Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data.
Spectroscopic Propertie: λexc 426 nm, λem 480 nm, ε 57.0 L mmol-1 cm-1 (Tris-HCl pH 7.5). |
Expiration Date | 12 months from date of receipt. |
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