DDX49 Antibody (C-term)

• Catalog Number: orb1927618
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23678
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54226 Da
• Target: This DDX49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-413 amino acids from the C-terminal region of human DDX49.
• UniProt ID: Q9Y6V7
• NCBI: NP_061943.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Probable ATP-dependent RNA helicase DDX49, DEAD bo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DDX49 Antibody (C-term) in Hela cell line lysates (35 ug/lane). DDX49 (arrow) was detected using the purified Pab.

DDX49 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

DDX49 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX49 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.