DDX3/DDX3X Antibody

• Catalog Number: orb402398
• Category: Antibodies
• Description: DDX3/DDX3X Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DDX3 (2-28aa SHVAVENALGLDQQFAGLDLNSSDNQS), identical to the related mouse sequence.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 73243 MW
• UniProt ID: O00571
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ATP-dependent RNA helicase DDX3X;3.6.4.13;DEAD box
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-DDX3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of ANA-1 cells using anti-DDX3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of C6 cells using anti-DDX3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of DDX3 using anti-DDX3 antibody.Lane 1:human HeLa cell; 2:human HepG2 cell; 3:human HT1080 cell; 4:rat liver tissue; 5:rat C6 cell; 6:mouse liver tissue; 7:mouse NIH/3T3 cell.

IF analysis of DDX3 using anti-DDX3 antibody. DDX3 was detected in an immunocytochemical section of SiHa cells.