DDX3 (phospho-Thr322) antibody

• Catalog Number: orb767859
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DDX3 (phospho-Thr322)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DDX3/DEAD-box Protein 3 around the phosphorylation site of Thr322. AA range:466-515
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-DDX3X antibody, anti-DBX antibody, anti-DDX3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody.

Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.