DDHD1 Antibody

• Catalog Number: orb1520494
• Category: Antibodies
• Description: DDHD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2-10 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: DDHD1
• UniProt ID: Q8NEL9
• NCBI: Q8NEL9.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: phospholipase DDHD1; phosphatidic acid-preferring
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human DDHD1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDHD1 antibody (orb1520494) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DDHD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDHD1 antibody (orb1520494) used for IP at 2 µg per reaction.