DCN monoclonal antibody (M01), clone 3H4-1F4

• Catalog Number: orb2293736
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant DCN.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: DCN (AAH05322, 1 a.a. ~ 359 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK
• Tested applications: ELISA, IHC-P, WB
• Clone Number: 3H4-1F4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH05322

Detection limit for recombinant GST tagged DCN is approximately 1 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to DCN on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]

Western Blot analysis of DCN expression in transfected 293T cell line by DCN monoclonal antibody (M01), clone 3H4-1F4. Lane 1: DCN transfected lysate (Predicted MW: 39.7 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (65.23 KDa).