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| Catalog Number | orb1946735 |
|---|---|
| Category | Assays and Kits |
| Description | DC(Deoxycholate) ELISA Kit |
| Range | 15.63-1000 ng/mL |
| Target | DC |
| Sensitivity | 4.99 ng/mL |
| Alternative names | Deoxycholic Acid; Cholanoic Acid; 3a,12a-dihydroxy Read more... |
| Note | For research use only |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Deoxycholate(DC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Deoxycholate(DC). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deoxycholate(DC) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
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