DC-SIGNR Antibody [16E7], Rabbit IgG

• Catalog Number: orb1672248
• Category: Antibodies
• Description: DC-SIGNR Antibody [16E7], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 16E7
• Tested applications: FC, IHC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: CD299
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CLEC4M
• UniProt ID: Q9H2X3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD299, C-type lectin domain family 4 member M, CD2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7. Jurkat cell extract (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of 16E7 (orb1672248) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa- tho ugh 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. orb1672248 successfully detected the canonical human DC-SIGNR- as well as multiple other isoforms.

Immunofluoresence staining of fixed HeLa cells with anti-DC-SIGNR antibody 16E7. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of 16E7 (orb1672248) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672248- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.