DBP A antibody

• Catalog Number: orb344662
• Category: Antibodies
• Description: DBP A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-A protein.
• Concentration: 1.06 mg/mL
• Dilution range: ELISA: 1:5,000, WB: 1:1,000
• Form/Appearance: Lyophilized
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi DbpA protein. A BLAST analysis was used to suggest cross-reactivity with DbpA from Borrelia burgdorferi sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Borrelia garinii sources based on 60-80% homology. Cross-reactivity with DbpA from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: O50917
• NCBI: NP_045697.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-DbpA Antibody, Decorin-binding Protein
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18.5 kDa in size corresponding to Borrelia burgdorferi DbpA protein by Western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Molecular weight markers MBP-DbpA fusion protein (arrow; expected MW: 60.9 kDa) using DBP A antibody