DBH antibody

• Catalog Number: orb12397
• Category: Antibodies
• Description: Goat polyclonal to DBH.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 69.1
• Target: DBH (aa437-448)
• Entrez: 1621
• Protein Sequence: DNHYSPHFQEIR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DBH antibody
• Note: For research use only
• Application notes: WB: Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 69.1kDa according to NP_000778.3). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particu. IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5µg/ml.
• Expiration Date: 12 months from date of receipt.

orb12397 (2µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb12397 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.