DBCO-PEG4-Biotin Conjugate

• Catalog Number: orb63989
• Category: Tools
• Description: DBCO-PEG 4-Biotin is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction. The hydrophilic PEG 4linker [22.8 Å (19 atoms)] reduces or elimi-nates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose. Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.
• CAS Number: 1255942-07-4
• Form/Appearance: off-white to slightly yellow solid at storage conditions, tends to become sticky amorphous solid; Color: off-white to slightly yellow
• Purity: ≥ 90% (HPLC)
• MW: Theoretical MW: 749.92 g/mol; Detected MW: 749.34 g/mol
• SMILES: N(CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)C(=O)CCOCCOCCOCCOCCNC(=O)CCCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2
• Formula: C₃₉H₅₁N₅O₈S
• Solubility (25°C): DMF, DMSO
• Storage: store at -20 °C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.