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Catalog Number | orb1523888 |
---|---|
Category | Antibodies |
Description | DBC1/p30 DBC Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 873 and 923 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | DBC1/p30 DBC |
UniProt ID | Q8N163 |
NCBI | NP_066997.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | DBC-1; p30DBC; p30 DBC protein; p30 DBC; NET35; KI Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human DBC1/p30 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody orb1523888 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of mouse DBC1/p30 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody orb1523888 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human DBC1/p30 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-DBC1/p30 antibody orb1523888 used for IP at 6 µg/mg lysate.
Detection of human DBC1/p30 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BC1/p30 (Cat. No. orb1523888) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of mouse DBC1/p30 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-BC1/p30 (Cat. No. orb1523888) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
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