Daxx Antibody

• Catalog Number: orb2635541
• Category: Antibodies
• Description: This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-DAXX-5G11
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human DAXX protein was used as the immunogen for the Daxx antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Daxx antibody is available for research use only.
• UniProt ID: Q9UER7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Daxx antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human thyroid tissue with Daxx antibody (clone PCRP-DAXX-5G11) at 2 ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-5G11), and unstained cells (red).

Immunofluorescent staining of PFA-fixed human HeLa cells using Daxx antibody (green, clone PCRP-DAXX-5G11) and phalloidin (red).

SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-5G11) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Daxx antibody (clone PCRP-DAXX-5G11). These results demonstrate the foremost specificity of the PCRP-DAXX-5G11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.