DARPP32/PPP1R1B Antibody

• Catalog Number: orb334612
• Category: Antibodies
• Description: DARPP32/PPP1R1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Predicted Reactivity: Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DARPP32 (1-36aa MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPA), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22963 MW
• UniProt ID: Q9UD71
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein phosphatase 1 regulatory subunit 1B;DARPP-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-DARPP32 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of PC-3 cells using anti-DARPP32 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of DARPP32 using anti-DARPP32 antibody.Lane 1:human CACO-2 cell; 2:rat brain tissue; 3:mouse brain tissue.

IHC analysis of DARPP32 using anti-DARPP32 antibody. DARPP32 was detected in a paraffin-embedded section of mouse pancreas tissue.

IHC analysis of DARPP32 using anti-DARPP32 antibody. DARPP32 was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of DARPP32 using anti-DARPP32 antibody. DARPP32 was detected in a paraffin-embedded section of human lung cancer tissue.