DARPP-32 (phospho Thr34) rabbit pAb

SKU: orb764398

Description

Rabbit polyclonal antibody to DARPP-32 (phospho-Thr34)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34. AA range:18-67
• Molecular Weight: 35
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-PPP1R1B antibody, anti-DARPP32 antibody, anti-Protein phosphatase 1 regulatory subunit 1B antibody, anti-DARPP-32 antibody, anti-Dopamine- and cAMP-regulated neuronal phosphoprotein antibody

Western Blot analysis of various cells using Phospho-DARPP-32 (T34) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of RAT-musle cells using Phospho-DARPP-32 (T34) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DARPP-32 (Phospho-Thr34) Antibody.

Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) Antibody. The picture on the right is blocked with the phospho peptide.