DAPK3 Antibody

• Catalog Number: orb1238532
• Category: Antibodies
• Description: DAPK3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.The immunogen is located within amino acids 270 - 320 of ZIPK.
• Concentration: 1 mg/ml
• Dilution range: ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26 kDa Observed: 27 kDa
• Target: DAPK3
• UniProt ID: O43293
• NCBI: BAA81746
• Storage: ZIPK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ZIPK Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ZIPK Antibody: ZIP, ZIPK, Death-associated protein
• Note: For research use only
• Application notes: ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 µg/mL.

Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 µg/mL.

Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 µg/mL.