DAPK1 (phospho-S736) antibody

• Catalog Number: orb515280
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DAPK1 (phospho-S736)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S736 of human DAP Kinase 1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: DAPK1
• Entrez: 69635, 1612
• UniProt ID: P53355, Q80YE7
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: DAPK; Death-associated protein kinase 1; DAP kinas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DAP Kinase 1 (Phospho-S736) expression in HEK293T (A), CT26 (B), NIH3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 160 kD; Observed band size: 160 kD)

Immunohistochemical analysis of DAP Kinase 1 (Phospho-S736) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.