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Catalog Number | orb2293780 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant DAPK1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2E7 |
Tested applications | ELISA, PLA |
Reactivity | Human |
Isotype | IgG1 kappa |
Immunogen | DAPK1 (NP_004929, 1211 a.a. ~ 1310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | LLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLT |
NCBI | NP_004929 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged DAPK1 is approximately 0.1 ng/ml as a capture antibody.
Proximity Ligation Analysis of protein-protein interactions between MAPK3 and DAPK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).