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Catalog Number | orb2293794 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant DAF. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1G3 |
Tested applications | ELISA, PLA, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | DAF (NP_000565, 35 a.a. ~ 134 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | DCGLPPDVPNAQPALEGRTSFPEDTVITYKCEESFVKIPGEKDSVICLRGSQWSDIEEFCNRSCEVPTRLNSASLKQPYITQNYFPVGTVVEYECRPGYR |
NCBI | NP_000565 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
DAF monoclonal antibody (M01), clone 1G3 Western Blot analysis of DAF expression in HeLa.
Detection limit for recombinant GST tagged DAF is approximately 0.03 ng/ml as a capture antibody.
Proximity Ligation Analysis of protein-protein interactions between LCK and CD55. HeLa cells were stained with anti-LCK rabbit purified polyclonal 1:1200 and anti-CD55 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot analysis of CD55 expression in transfected 293T cell line by DAF monoclonal antibody (M01), clone 1G3. Lane 1: CD55 transfected lysate (41.4 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (36.74 KDa).