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Catalog Number | orb1524606 |
---|---|
Category | Antibodies |
Description | DACH1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | between 740 and 760 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | DACH1 |
UniProt ID | Q9UI36 |
NCBI | CAH73005.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Dach1; dachshund homolog 1; dac homolog; DACH Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human DACH1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DACH1 antibody orb1524606 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. DACH1 was also immunoprecipitated by rabbit anti-DACH1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human DACH1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DACH1 (Cat. No. orb1524606) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
WB | |
Human, Mouse, Porcine, Yeast | |
Human, Mouse, Porcine, Yeast | |
Rabbit | |
Polyclonal | |
Unconjugated |
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