DAB Chromogen Kit

SKU: orb219876

Description

3,3'-Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2.Reactions using diaminobenzidine (DAB) to localize the enzyme peroxidase in neutrophils and peroxidase-antiperoxidase (PAP) complex during immunological staining are usually performed in Tris-HCl or phosphate buffer at pH 7.2-7.6. However, DAB solutions at pH 7.2-7.6 often demonstrate erythrocyte pseudoperoxidase as well. By lowering the pH of the DAB solutions, it is possible to selectively suppress the reactivity of pseudoperoxidase while maintaining optimal reactions in neutrophils and PAP complex. This derivative of benzidine is a precursor to polybenzimidazole, which forms fibers that are renown for their chemical and thermal stability.DAB (3,3'-diaminobenzidine) is oxidized in the presence of peroxidase and hydrogen peroxide resulting in the deposition of a brown, alcohol-insoluble precipitate at the site of enzymatic activity.DAB (3, 3

Storage & Handling

• Storage: Store at 4°C for one year.
• Disclaimer: For research use only

Immunohistochemical analysis staining in human breast carcinoma formalin fixed paraffin-embedded tissue section. The section was detected using DAB Chromogen Kit. The section was then counterstained with haematoxylin and mounted with Neutral Gum.