D17Wsu104e Antibody

• Catalog Number: orb1272706
• Category: Antibodies
• Description: D17Wsu104e Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSF-20. Murine SF-20 specific antibody was purified by affinity chromatography employing immobilized mSF-20 matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect mSF-20 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.SandwichTo detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine SF-20 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Western Blot To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: D17Wsu104e
• UniProt ID: Q9CPT4
• NCBI: Q9CPT4
• Storage: SF-20 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Il25, Ly6elg, Il25, UPF0556 protein C19orf10 homol
• Note: For research use only
• Application notes: ELISA:Indirect:To detect mSF-20 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.SandwichTo detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine SF-20 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Western Blot To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect mSF-20 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine SF-20 (orb1272705) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.

To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.