Cytokeratin, pan (Epithelial Marker) Antibody

• Catalog Number: orb2317321
• Category: Antibodies
• Description: Cytokeratin, pan (Epithelial Marker) Antibody
• Target: KRT77
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Immunogen: Recombinant fragments and/or synthetic peptides of human Cytokeratin proteins (exact sequences are proprietary)
• UniProt ID: Q7Z794, Q01546
• MW: 40-67kDa (Multiple)
• Tested applications: FC, IF, IHC, WB
• Dilution range: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (0.5-2ug/ml for 2 hours at RT);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: HeLa or MCF-7 cells. Human skin, adeno- or squamous carcinomas.
• Antibody Type: Recombinant Antibody
• Clone Number: MonoPoly/4999R
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KRT1, KRT3, KRT4, KRT5, KRT6, KRT7, KRT8, KRT10, K
• Note: For research use only
• Entrez: 51350, 76, 77, 374454