Cytokeratin 7/17 antibody

• Catalog Number: orb43713
• Category: Antibodies
• Description: Mouse Monoclonal to Cytokeratin 7/17.
• Clonality: Monoclonal
• Clone Number: C-46
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Bovine, Human, Porcine
• Isotype: Mouse IgG1
• Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB; positive tissue: pancreas.Western blotting: Recommended dilution: 1-2 μg/ml; positive control: HeLa cells.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Cytokeratin 7/17
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Cytokeratin 7+17 antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB; positive tissue: pancreas.Western blotting: Recommended dilution: 1-2 μg/ml; positive control: HeLa cells.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7+17 (C-46).

Immunohistochemistry staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).

Western blotting analysis of human cytokeratin 7+17 using mouse monoclonal antibody C-46 on lysates of HeLa cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 7+17 monoclonal antibody C-46 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for cytokeratin 17 at approximately 46 kDa and for cytokeratin 7 at approximately 54 kDa.